Demo construct
Synthetic circular demonstration construct. The CDS encodes the made-up peptide MAEELKGENHELIDEMAPEPTIDE. The promoter landmark is the published T7 consensus. This is not a natural plasmid and not a cloning recipe.
3D sequence
Fiber draws the duplex the way a stained molecule reads under a microscope: two continuous backbones and base-pair rungs. 10.5 bp per turn. Position 1 is marked. Each rung is one base pair. The outer ribbon is GC, arcs are annotations, inner lines are open reading frames, and amber marks are restriction sites. This is a drawing of the string, not an atomic model.
Search runs on this sequence, including across the origin when the molecule is circular.
Digest, guides, primers, codon choice, assembly, and the population path all use this project. Pass a result and the map, the working sequence, and Compare pick it up.
Restriction digest
267 enzymes from TeselaGen sequence-utils. Overlap with GATC or CCWGG is flagged. That is a sequence fact, not a prediction that the enzyme is blocked.
- EcoRI · forward7–12 · overhang AATT
- BamHI · forward · overlaps GATC192–197 · overhang GATC
Fragments
- 185 bpEcoRI → BamHI · 8–192
- 29 bpBamHI → EcoRI · 193–7