3D sequence
Fiber draws the duplex the way a stained molecule reads under a microscope: two continuous backbones and base-pair rungs. 10.5 bp per turn. Position 1 is marked. Each rung is one base pair. The outer ribbon is GC, arcs are annotations, inner lines are open reading frames, and amber marks are restriction sites. This is a drawing of the string, not an atomic model.
Search runs on this sequence, including across the origin when the molecule is circular.
Digest, guides, primers, codon choice, assembly, and the population path all use this project. Pass a result and the map, the working sequence, and Compare pick it up.
Primer pairs
Melting temperature is SantaLucia 1998 at Primer3’s default salt, from TeselaGen sequence-utils. Pair choice is local and does not call primer3_core. A 3′ dimer is four complementary bases at the ends. A hairpin is a stem of at least four with a loop of three to eight. Neither is a ΔG score.
No pair passed the length, GC, and Tm filters for this interval.